This specification defines the basic principles, requirements and methods of bacterial reverse mutation test. The specification applies to the detection of gene mutation for cosmetic raw materials and products.

This specification consists of 11 parts as listed below:

1. Scope

2. Definition

3. Principle

4. Instruments and Equipment

5. Medium and Reagents

6. Identification of Test Strains and Their Biological Characteristics

7. Induction of Rat Liver Licrosomal Enzymes and Preparation of S9

8. Choice of Solvent

9. Dosage Design

10. Test Operation Steps

11. Data Processing and Result Judgment