This method, also known as In Vitro 3T3 NRU Phototoxicity Test for Chemicals in Cosmetics, is included as the 18^th toxicology test method into Chapter 6 of Safety and Technical Standards for Cosmetics 2015. It specifies the scope, normative references, terms and definitions, test principle, test materials and reagents, test procedures and result judgment criteria of in-vitro 3T3 neutral red uptake phototoxicity test for chemicals used in cosmetics. It is recommended that this method applies to the evaluation of the potential phototoxicity of chemicals used in cosmetics.

The main content of this method are as follows:

1. Scope

2. Normative References

3. Terms and Definitions

4. Test Principle

5. Test Materials and Reagents

6. Test Procedures

7. Results Judgment Criteria

• Annex A NRU PT Test Flow Chart

• Annex B A Schematic Diagram for Sample Injection in 96-well Plate

• Annex C Spectral Power of Solar Simulator and Sensitivity of Cells